Bioremediation: A Sustainable Approach for Environmental Cleanup 3

as bioaugmentation involves the use of different cultured microorganisms to degrade soil and

water contaminants. Microorganisms must enzymatically attack contaminants and transform them

into harmless compounds for bioremediation to be efficient (Vidali 2001). Since environmental

conditions must be favorable for microbial development for bioremediation to be effective,

environmental parameters are regulated/monitored to accelerate microbial growth and deterioration.

Bioremediation procedures are often less expensive than traditional methods like incineration, and

certain contaminants may be treated on-site, lowering exposure hazards for cleanup workers and

possibly broader exposure due to transportation problems. Bioremediation is more widely accepted

than other approaches since it is based on natural attenuation. Most bioremediation systems are

operated under aerobic circumstances; however, operating one under anaerobic conditions may

allow microbial organisms to degrade compounds that are typically resistant to degradation (Colberg

and Young 1995).

1.4 Types of Bioremediation

The bioremediation process is divided into in-situ and ex-situ based on the origin and removal of

pollutants, as shown in Figure 1.1.

In situ

Ex situ

Figure 1.1. Types of bioremediation techniques (Tyagi and Kumar 2021).

1.4.1 In-situ Bioremediation

In-situ bioremediation is defined as the process of degrading the contaminants in a naturalistic

environment to produce carbon dioxide and water. It is a low-cost, low-maintenance, environmentally

beneficial and long-term solution for contaminated site cleaning (Aggarwal et al. 1990, Jørgensen,

2007, Megharaj et al. 2011, Latha and Reddy 2013). In-situ bioremediation is most commonly

used to degrade pollutants in saturated soils and groundwater. This technique is basically applied

to polluted material on-site. Some of the examples of in-situ bioremediation strategies as shown in

Figure 1.1 are described next.